Gametogenesis and Early Embryo Development

Publications

The problem lies in the medium: challenges for diagnostic use of spent culture media in IVF

Frontiers in Reproductive Health

April 2026 | Opinion

Zuzana Holubcová

 

The quest for metabolic biomarkers of IVF outcomes: a meta-analysis and critical review

Frontiers in Cell and Developmental Biology

October 2025 | Review article

Pavel Šmak; Jan Juřica; Jana Gregorová; Volodymyr Porokh; Ondřej Peš; Zuzana Holubcová

 

Non-standardized protein background in IVF media linked to serum-derived albumin supplementation

Journal of Assisted Reproduction and Genetics

October 2025 | Journal article

Markéta Nezvedová; Volodymyr Porokh; Tami Bočková; Václav Pustka; Drahomíra Kyjovská; Barbora Maierová; Soňa Kloudová; Pavel Otevřel; Zuzana Holubcová

 

Electron Microscopy of Human Oocytes

Methods in molecular biology

July 2025 | Protocol

Zuzana Holubcová; Barbora Baďurová; Zuzana Trebichalská

 

Female-age-dependent changes in the lipid fingerprint of the mammalian oocytes

Human Reproduction

October 2024 | Journal article

Simona Bisogno; Joanna Depciuch; Hafsa Gulzar; Maria Florencia Heber; Michał Kobiałka; Łukasz Gąsior; Adrianna Bereta; Anna Pieczara; Kinga Fic; Richard Musson; Gabriel Garcia Gamero; Maria Pardo Martinez; Alba Fornés Pérez; Martina Tatíčková; Zuzana Holubcova; Małgorzata Barańska; Grażyna Ewa Ptak

 

Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos

Nature Communications

July 2024 | Journal article

Volodymyr Porokh; Drahomíra Kyjovská; Martina Martonová; Tereza Klenková; Pavel Otevřel; Soňa Kloudová; Zuzana Holubcová

 

The ultrastructural nature of human oocytes’ cytoplasmic abnormalities and the role of cytoskeleton dysfunction

F&S Science

November 2023 | Journal article

Martina Tatíčková; Zuzana Trebichalská; Drahomíra Kyjovská; Pavel Otevřel; Soňa Kloudová; Zuzana Holubcová

 

Actin-driven chromosome clustering facilitates fast and complete chromosome capture in mammalian oocytes

Nature Cell Biology

February 2023 | Journal article

Katarina Harasimov; Julia Uraji; Eike Urs Mönnich; Zuzana Holubcová; Kay Elder; Martyn Blayney; Melina Schuh

 

MAIA, Fc receptor–like 3, supersedes JUNO as IZUMO1 receptor during human fertilization

Science Advances

September 2022 | Journal article

Zuzana Holubcová

 

Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells

Tissue Engineering and Regenerative Medicine

June 2022 | Journal article

Hana Kotasová; Michaela Capandová; Vendula Pelková; Jana Dumková; Zuzana Koledová; Ján Remšík; Karel Souček; Zuzana Garlíková; Veronika Sedláková; Anas Rabata; Petr Vaňhara; Lukáš Moráň; Lukáš Pečinka; Volodymyr Porokh; Martin Kučírek; Libor Streit; Josef Havel & Aleš Hampl

 

High-Resolution 3D Reconstruction of Human Oocytes Using Focused Ion Beam Scanning Electron Microscopy

Frontiers in Cell and Developmental Biology

November 2021 | Journal article

Zuzana Trebichalská; Jakub Javurek; Martina Tatícková; Drahomíra Kyjovská; Sona Kloudová; Pavel Otevrel; Aleš Hampl and Zuzana Holubcová

 

Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

Stem Cell Research

October 2021 | Journal Article

Tereza Souralova; Zuzana Holubcova; Drahomira Kyjovska; Ales Hampl; Irena Koutna

 

Live birth achieved despite the absence of ejaculated spermatozoa and mature oocytes retrieved: a case report

Journal of Assisted Reproduction and Genetics

April 2021 | Journal article

Zuzana Holubcová; Pavel Otevřel; Marek Koudelka & Soňa Kloudová

 

Cytoplasmic maturation in human oocytes: an ultrastructural study †

Biology of Reproduction

September 2020 | Journal article

Zuzana Trebichalská; Drahomíra Kyjovská; Soňa Kloudová; Pavel Otevrel; Aleš Hampl and Zuzana Holubcová

 

Perfect date—the review of current research into molecular bases of mammalian fertilization

Journal of Assisted Reproduction and Genetics

February 2020 | Literature review

Zuzana Trebichalská & Zuzana Holubcová

 

Human Egg Maturity Assessment and Its Clinical Application

Journal of Visualized Experiments

August 2019 | Journal article

Zuzana Holubcová; Drahomíra Kyjovská; Martina Martonová; Darja Páralová; Tereza Klenková; Soňa Kloudová

 

FSH administration affects amino acid metabolism in mammalian oocytes

Biology of Reproduction

July 2019 | Journal article

Anna Tetkova; Andrej Susor; Michal Kubelka; Lucie Nemcova; Denisa Jansova; Michal Dvoran; Edgar Del Llano; Zuzana Holubcova; Jaroslav Kalous; Author Notes

 

Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes

Journal of Assisted Reproduction and Genetics

March 2019 | Journal article

Zuzana Holubcová; Drahomíra Kyjovská; Martina Martonová; Darja Páralová; Tereza Klenková; Pavel Otevřel; Radka Štěpánová; Soňa Kloudová & Aleš Hampl

 

Generation of human iPSCs from fetal prostate fibroblasts HPrF

Stem Cell Research

March 2019 | Journal article

Zuzana Kahounová; Eva Slabáková; Lucia Binó; Ján Remšík; Radek Fedr; Jan Bouchal; Radek Vrtěl; Lucie Jurečková; Volodymyr Porokh; Darja Páralová; Aleš Hampl; Karel Souček

 

Presence of growth/differentiation factor-15 cytokine in human follicular fluid, granulosa cells, and oocytes

Journal of Assisted Reproduction and Genetics

August 2018 | Journal article

Karel Souček; Alice Malenovská; Zuzana Kahounová; Ján Remšík; Zuzana Holubcová; Tomáš Soukup; Daniela Kurfürstová; Jan Bouchal; Tereza Suchánková; Eva Slabáková & Aleš Hampl

 

Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A

Stem Cell Research

December 2018 | Journal article

Zuzana Kahounová; Eva Slabáková; Lucia Binó; Ján Remšík; Radek Fedr; Jan Bouchal; Daniela Kurfűrstová; Radek Vrtěl; Vladimír Študent; Lucie Jurečková; Volodymyr Porokh; Aleš Hampl; Karel Souček

 

Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells

Stem Cells and Development

June 2018 | Journal article

Volodymyr Porokh; Petr Vaňhara; Tomáš Bárta; Lucie Jurečková; Dáša Bohačiaková; Veronika Pospíšilová; Daniela Mináriková; Zuzana Holubcová; Vendula Pelková; Karel Souček; and Aleš Hampl

 

Sister kinetochore splitting and precocious disintegration of bivalents could explain the maternal age effect

eLife

December 2015 | Journal article

Agata P Zielinska; Zuzana Holubcova; Martyn Blayney; Kay Elder; Melina Schuh

 

Error-Prone Chromosome-Mediated Spindle Assembly Favors Chromosome Segregation Defects in Human Oocytes

Obstetrical and Gynecological Survey

September 2015 | Journal article

Zuzana Holubcová; Martzn Blayney; Kay Elder; Melina Schuh

 

Human oocytes. Error-prone chromosome-mediated spindle assembly favors chromosome segregation defects in human oocytes

Science

June 2015 | Journal article

Zuzana Holubcová; Martyn Blayney; Kay Elder; and Melina Schuh

 

Vesicles modulate an actin network for asymmetric spindle positioning

Nature Cell Biology

July 2013 | Journal article

Zuzana Holubcová; Gillian Howard & Melina Schuh

 

Cell cycle regulation in human embryonic stem cells: Links to adaptation to cell culture

Experimental Biology and Medicine

March 2013 | Literature review

Tomas Barta; Dasa Dolezalova; Zuzana Holubcova; and Ales Hampl

 

 

MicroRNAs Regulate p21Waf1/Cip1 Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells

Stem Cells

July 2012 | Journal article

Dasa Dolezalova; Marek Mraz; Tomas Barta; Karla Plevova; Vladimir Vinarsky; Zuzana Holubcova; Josef Jaros; Petr Dvorak; Sarka Pospisilova; Ales Hampl

 

Human Embryonic Stem Cells Suffer from Centrosomal Amplification

Stem Cells

January 2011 | Journal article

Zuzana Holubcová; Pavel Matula; Miroslava Sedláčková; Vladimír Vinarský; Dáša Doležalová; Tomáš Bárta; Petr Dvořák; Aleš Hampl

 

 

Human Embryonic Stem Cells Are Capable of Executing G1/S Checkpoint Activation

Stem Cells

July 2010 | Journal article

Tomáš Bárta; Vladimír Vinarský; Zuzana Holubcová; Dáša Doležalová; Jan Verner; Šárka Pospíšilová; Petr Dvořák; Aleš Hampl

 

Lineage specific composition of cyclin D–CDK4/CDK6–p27 complexes reveals distinct functions of CDK4, CDK6 and individual D-type cyclins in differentiating cells of embryonic origin

Cell Proliferation

January 2009 | Journal article

V. Bryja; J. Pacherník; J. Vondrácek; K. Soucek; L. Cajánek; V. Horvath; Z. Holubcová; P. Dvorák; and A. Hampl

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