Publications

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2024

Hruska-Plochan M, Wiersma VI, Betz KM, Mallona I, Ronchi S, Maniecka Z, Hock EM, Tantardini E, Laferriere F, Sahadevan S, Hoop V, Delvendahl I, Pérez-Berlanga M, Gatta B, Panatta M, van der Bourg A, Bohaciakova D, Sharma P, De Vos L, Frontzek K, Aguzzi A, Lashley T, Robinson MD, Karayannis T, Mueller M, Hierlemann A, Polymenidou M. A model of human neural networks reveals NPTX2 pathology in ALS and FTLD. Nature. 2024 Feb;626(8001):1073-1083. doi: 10.1038/s41586-024-07042-7. Epub 2024 Feb 14. PMID: 38355792; PMCID: PMC10901740.
Kubickova B, Martinkova S, Bohaciakova D, Hilscherova K. Cyanobacterial anatoxin-a does not induce in vitro developmental neurotoxicity, but changes gene expression patterns in co-exposure with all-trans retinoic acid. Toxicol Lett. 2024 Jan;391:39-44. doi: 10.1016/j.toxlet.2023.12.004. Epub 2023 Dec 7. PMID: 38070836.

2023

Kadakova S, Raska J, Hribkova H, Fedorova V, Sedmik J, Plesingrova K, Sheardova K, Bohaciakova D. Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-, Age-, and ApoE-Matched Healthy Control. Stem Cell Res. 2023 Dec 7;74:103273. doi: 10.1016/j.scr.2023.103273. Epub ahead of print. PMID: 38100913.
Vanova T, Sedmik J, Raska J, Amruz Cerna K, Taus P, Pospisilova V, Nezvedova M, Fedorova V, Kadakova S, Klimova H, Capandova M, Orviska P, Fojtik P, Bartova S, Plevova K, Spacil Z, Hribkova H, Bohaciakova D. Cerebral organoids derived from patients with Alzheimer's disease with PSEN1/2 mutations have defective tissue patterning and altered development. Cell Rep. 2023 Nov 28;42(11):113310. doi: 10.1016/j.celrep.2023.113310. Epub 2023 Oct 20. PMID: 37864790.
Celiker C, Weissova K, Cerna KA, Oppelt J, Dorgau B, Gambin FM, Sebestikova J, Lako M, Sernagor E, Liskova P, Barta T. Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light. iScience. 2023 Jun 28;26(7):107237. doi: 10.1016/j.isci.2023.107237. PMID: 37485345; PMCID: PMC10362355.
Jensen AM, Raska J, Fojtik P, Monti G, Lunding M, Vochyanova S, Pospisilova V, van der Lee SJ, Van Dongen J, Bossaerts L, Van Broeckhoven Ch, Dols O, Lleo A, Benussi L, Ghidoni R, Hulsman M, Sleegers K, Bohaciakova D, Holstege H, Andersen O. The SORL1 p.Y1816C variant causes impaired endosomal dimerization and autosomal dominant Alzheimer's disease. preprint doi: https://doi.org/10.1101/2023.07.09.23292253
Nemergut M, Marques SM, Uhrik L, Vanova T, Nezvedova M, Gadara DC, Jha D, Tulis J, Novakova V, Planas-Iglesias J, Kunka A, Legrand A, Hribkova H, Pospisilova V, Sedmik J, Raska J, Prokop Z, Damborsky J, Bohaciakova D, Spacil Z, Hernychova L, Bednar D, Marek M. Domino-like effect of C112R mutation on ApoE4 aggregation and its reduction by Alzheimer's Disease drug candidate. Mol Neurodegener. 2023 Jun 6;18(1):38. doi: 10.1186/s13024-023-00620-9. PMID: 37280636; PMCID: PMC10245438.
Fedorova V, Pospisilova V, Vanova T, Amruz Cerna K, Abaffy P, Sedmik J, Raska J, Vochyanova S, Matusova Z, Houserova J, Valihrach L, Hodny Z, Bohaciakova D. Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration. Mol Oncol. 2023 Apr;17(4):647-663. doi: 10.1002/1878-0261.13389. Epub 2023 Feb 18. PMID: 36744875; PMCID: PMC10061278.
Kubickova B, Martinkova S, Bohaciakova D, Nezvedova M, Liu R, Brozman O, Spáčil Z, Hilscherova K. Effects of all-trans and 9-cis retinoic acid on differentiating human neural stem cells in vitro. Toxicology. 2023 Mar 15;487:153461. doi: 10.1016/j.tox.2023.153461. Epub 2023 Feb 16. PMID: 36805303; PMCID: PMC10019519.
Fedorova V, Amruz Cerna K, Oppelt J, Pospisilova V, Barta T, Mraz M, Bohaciakova D. MicroRNA Profiling of Self-Renewing Human Neural Stem Cells Reveals Novel Sets of Differentially Expressed microRNAs During Neural Differentiation In Vitro. Stem Cell Rev Rep. 2023 Mar 14. doi: 10.1007/s12015-023-10524-2. Epub ahead of print. PMID: 36918496.
Nezvedova M, Jha D, Vanova T, Gadara D, Klimova H, Raska J, Opalka L, Bohaciakova D, Spacil Z. Single Cerebral Organoid Mass Spectrometry of Cell-Specific Protein and Glycosphingolipid Traits. Anal Chem. 2023 Feb 14;95(6):3160-3167. doi: 10.1021/acs.analchem.2c00981. Epub 2023 Feb 1. PMID: 36724094; PMCID: PMC10016744.

2022

Barak M, Fedorova V, Pospisilova V, Raska J, Vochyanova S, Sedmik J, Hribkova H, Klimova H, Vanova T, Bohaciakova D. Human iPSC-Derived Neural Models for Studying Alzheimer's Disease: from Neural Stem Cells to Cerebral Organoids. Stem Cell Rev Rep. 2022 Feb;18(2):792-820. doi: 10.1007/s12015-021-10254-3. Epub 2022 Feb 2. PMID: 35107767; PMCID: PMC8930932.
Pospisil J, Hrabovsky M, Bohaciakova D, Hovadkova Z, Jurasek M, Mlcouskova J, Paruch K, Nevolova S, Damborsky J, Hampl A, Jaros J. Geometric Control of Cell Behavior by Biomolecule Nanodistribution. ACS Biomater Sci Eng. 2022 Nov 14;8(11):4789-4806. doi: 10.1021/acsbiomaterials.2c00650. Epub 2022 Oct 6. PMID: 36202388; PMCID: PMC9667466.

2021

Capkova N, Pospisilova V, Fedorova V, Raska J, Pospisilova K, Dal Ben M, Dvorak A, Viktorova J, Bohaciakova D, Vitek L. The Effects of Bilirubin and Lumirubin on the Differentiation of Human Pluripotent Cell-Derived Neural Stem Cells. Antioxidants (Basel). 2021 Sep 27;10(10):1532. doi: 10.3390/antiox10101532. PMID: 34679668; PMCID: PMC8532948.
Sucha R, Kubickova M, Cervenka J, Hruska-Plochan M, Bohaciakova D, Vodickova Kepkova K, Novakova T, Budkova K, Susor A, Marsala M, Motlik J, Kovarova H, Vodicka P. Targeted mass spectrometry for monitoring of neural differentiation. Biology open 10 (8), bio058727
Raska J, Klimova H, Sheardova K, Fedorova V, Hribkova H, Pospisilova V, Vochyanova S, Vanova T, Bohaciakova D.* Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer’s disease and three sex-and age-matched healthy controls. Stem Cell Research 53, 102378 *Corresponding author
Raska J, Hribkova H, Klimova H, Fedorova V, Barak M, Barta T, Pospisilova V, Vochyanova S, Vanova T, Bohaciakova D.* Generation of six human iPSC lines from patients with a familial Alzheimer’s disease (n= 3) and sex-and age-matched healthy controls (n= 3). Stem Cell Research 53, 102379 *Corresponding author
Barta T, Bohaciakova D, Cajanek L. Self-renewal in induced pluripotent stem cells. Recent Advances in iPSC Technology, 179-207 Book chapter

2020

Vecera J, Prochazkova J, Sumberova V, Panska V, Paculova H, Kohutkova Lanova M, Masek J, Bohaciakova D, Andersson ER, Pachernik J. Hypoxia/Hif1α prevents premature neuronal differentiation of neural stem cells through the activation of Hes1. Stem cell research 45, 101770

2019

Almenar-Queralt A, Merkurjev D, Kim HS, Navarro M, Qi Ma, Chaves RS, Allegue C, Driscoll SP, Chen AG, Kohlnhofer B, Fong LK, Woodruff G, Mackintosh C, Bohaciakova D, Hruska-Plochan M, Tadokoro T, Young JE, Hajj NE, Dittrich M, Marsala M, Goldstein LSB, Garcia-Bassets I. Chromatin establishes an immature version of neuronal protocadherin selection during the naive-to-primed conversion of pluripotent stem cells. Nature Genetics 51 (12), 1691-1701
Bohaciakova D, Hruska-Plochan M, Tsunemoto R, Gifford WD, Driscoll SP, Glenn TD, Wu S, Marsala S, Navarro M, Tadokoro T, Juhas S, Juhasova J, Platoshyn O, Piper D, Sheckler V, Ditsworth D, Pfaff LS, Marsala M. A scalable solution for isolating human multipotent clinical-grade neural stem cells from ES precursors. Stem cell research & therapy 10 (1), 1-19
Fedorova V, Vanova T, Elrefae L, Pospisil J, Petrasova M, Kolajova V, Hudacova Z, Baniariova J, BarakN M, Peskova L, Barta T, Kaucka M, Killinger M, Vecera J, Bernatik O, Cajanek L, Hribkova H, Bohaciakova D.* Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of …Stem cell research 40, 101563 *Corresponding author

2018

Renzova T, Bohaciakova D, Esner M, Pospisilova V, Barta T, Hampl A, Cajanek L. Inactivation of PLK4-STIL module prevents self-renewal and triggers p53-dependent differentiation in human pluripotent stem cells. Stem cell reports 11 (4), 959-972
Porokh V, Vanhara P, Barta T, Jureckova L, Bohaciakova D, Pospisilova V, Minarikova D, Holubcova Z, Pelkova V, Soucek K, Hampl A. Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells. Stem cells and development 27 (16), 1077-1084
Strnadel J, Carromeu C, Bardy C, Navarro M, Platoshyn O, Glud AN, Marsala S, Kafka J, Miyanohara A, Kato T Jr., Tadokoro T, Hefferan MP, Kamizato K, Yoshizumi T, Juhas S, Juhasova J, Ho CS, Kheradmand T, Chen P, Bohaciakova D, Hruska-Plochan M, Todd AJ, Driscoll SP, Glenn TD, Pfaff SL, Klima J, Ciacci J, Curtis C, Gage FH, Bui J, Yamada K, Muotri AR and Marsala M Survival of syngeneic and allogeneic iPSC–derived neural precursors after spinal grafting in minipigs. Science translational medicine 10 (440)

2017

Bohaciakova D*, Renzova T, Fedorova V, Barak M, Kunova Bosakova M, Hampl A, Cajanek L*. An efficient method for generation of Knock-out human embryonic stem cells using CRISPR/Cas9 system. Stem Cells Dev. 2017 Nov 1. *Corresponding authors

Bagherpoor AJ, Dolezalova D*, Barta T, Kucirek M, Sani SA, Esner M, Bosakova MK, Vinarsky V, Peskova L, Hampl A, Stros M*. Properties of Human Embryonic Stem Cells and their Differentiated Derivatives Depend on Non-histone DNA-Binding HMGB1 and HMGB2 Proteins. Stem Cells Dev. 2017 Mar 1. PubMed PMID: 27863459; *Corresponding authors

Varun D, Srinivasan GR, Tsai YH, Kim HJ, Cutts J, Petty F, Merkley R, Stephanopoulos N, Dolezalova D, Marsala M, Brafman DA. A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs). Acta Biomaterialia. 2017 Jan 15. http://dx.doi.org/10.1016/j.actbio.2016.10.037

2015

Juhasova J, Juhas S, Hruska-Plochan M, Dolezalova D, Holubova M, Strnadel J, Marsala S, Motlik J, Marsala M. Time course of spinal doublecortin expression in developing rat and porcine spinal cord: implication in in vivo neural precursor grafting studies. Cellular and molecular neurobiology. 2015 Jan 1. doi:10.1007/s10571-014-0145-7

Dolezalova D, Mraz M, Hampl A. MicroRNAs in Embryonic Stem Cells. Book chapter. In: MicroRNA in Regenerative Medicine. Published by Elsevier. http://dx.doi.org/10.1016/B978-0-12-405544-5.00007-1

2014

Dolezalova D, Hruska-Plochan M, Bjarkam CR, Sørensen JC, Cunningham M, Weingarten D, Ciacci JD, Juhas S, Juhasova J, Motlik J, Hefferan MP, Hazel T, Johe K, Carromeu C, Muotri A, Bui J, Strnadel J, Marsala M. Pig models of neurodegenerative disorders: Utilization in cell replacement-based preclinical safety and efficacy studies. J Comp Neurol. 2014 Aug 15;522(12):2784-801. doi: 10.1002/cne.23575. Review. PubMed PMID: 24610493.

2013

Barta T, Dolezalova D, Holubcova Z, Hampl A. Cell cycle regulation in human embryonic stem cells: links to adaptation to cell culture. Exp Biol Med (Maywood). 2013 Mar;238(3):271-5. doi: 10.1177/1535370213480711. Review. PubMed PMID: 23598972.

2012

Dolezalova D, Mraz M, Barta T, Plevova K, Vinarsky V, Holubcova Z, Jaros J, Dvorak P, Pospisilova S, Hampl A. MicroRNAs regulate p21(Waf1/Cip1) protein expression and the DNA damage response in human embryonic stem cells. Stem Cells. 2012 Jul;30(7):1362-72. doi: 10.1002/stem.1108. PubMed PMID: 22511267.

Mraz M, Dolezalova D, Plevova K, Stano Kozubik K, Mayerova V, Cerna K, Musilova K, Tichy B, Pavlova S, Borsky M, Verner J, Doubek M, Brychtova Y, Trbusek M, Hampl A, Mayer J, Pospisilova S. MicroRNA-650 expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia. Blood. 2012 Mar 1;119(9):2110-3. doi: 10.1182/blood-2011-11-394874. PubMed PMID: 22234685.

2011

Holubcova Z, Matula P, Sedlackova M, Vinarsky V, Dolezalova D, Barta T, Dvorak P, Hampl A. Human embryonic stem cells suffer from centrosomal amplification. Stem Cells. 2011 Jan;29(1):46-56. doi: 10.1002/stem.549. PubMed PMID: 20960514.

2010

Barta T, Vinarsky V, Holubcova Z, Dolezalová D, Verner J, Pospisilova S, Dvorak P, Hampl A. Human embryonic stem cells are capable of executing G1/S checkpoint activation. Stem Cells. 2010 Jul;28(7):1143-52. doi: 10.1002/stem.451. PubMed PMID: 20518019.

Borghese L, Dolezalova D, Opitz T, Haupt S, Leinhaas A, Steinfarz B, Koch P, Edenhofer F, Hampl A, Brüstle O. Inhibition of notch signaling in human embryonic stem cell-derived neural stem cells delays G1/S phase transition and accelerates neuronal differentiation in vitro and in vivo. Stem Cells. 2010 May;28(5):955-64. doi: 10.1002/stem.408. PubMed PMID: 20235098.