Petr Vaňhara Research group: Tissue plasticity and regeneration
We are curious how:
- tissue microenvironment promotes changes cell and lineage specifications in normal and cancer tissues
- individual integrated cell stress response affect cancer and stem cell phenotypes
- molecular machinery of endoplasmic reticulum and unfolded protein response alters tissue functions and repair
- state-of-the-art microscopy including super resolution and live-cell imaging
- advanced 3D cultures
- range of molecular, bioanalytical and histological methods and tools
We develop techniques for:
- cell profiling and phenotyping using mass spectrometry and chemometrics
- mass spectrometry-assisted tissue analysis and diagnostics
Our lab primarily focuses on the role of endoplasmic reticulum (ER) in plasticity and regeneration of normal and diseased epithelial tissues. We investigate how the canonical and non-canonical ER signaling alters tissue response to various form of cell stress, and how this response alters tissue morphology and functions.
We have introduced dedicated tissue models to reveal fine mechanisms of tissue regeneration and plasticity. In particular, it is the ovarian surface epithelium, endometrial stromal cells or lung cells derived from pluripotent stem cells. To address research questions we use the state-of-the-art microscopy including super resolution and live-cell imaging and range of molecular, bioanalytical and histological methods and tools. In addition, we develop bionalytical techniques for cell profiling and phenotyping using whole-cell mass spectrometry and chemometrics.